SnapShot: The Replisome

SnapShot: The Replisome

Replisome-mediated DNA replication.

The elaborate process of genomic replication requires a large collection of proteins properly assembled at a DNA replication fork. Several decades of research on the bacterium Escherichia coli and its bacteriophages T4 and T7 have defined the roles of many proteins central to DNA replication. These three different prokaryotic replication systems use the same fundamental components for synthesis...

The Eukaryotic Replisome Goes Under the Microscope

The machinery at the eukaryotic replication fork has seen many new structural advances using electron microscopy and crystallography. Recent structures of eukaryotic replisome components include the Mcm2-7 complex, the CMG helicase, DNA polymerases, a Ctf4 trimer hub and the first look at a core replisome of 20 different proteins containing the helicase, primase, leading polymerase and a laggin...

The Novel Coronavirus – A Snapshot of Current Knowledge

Another animal to human transmission of a coronavirus occurred in December 2019 on a live animal market in the Chinese city of Wuhan causing an epidemic in China, reaching now different continents. This minireview summarizes the research literature on the virological, clinical and epidemiological aspects of this epidemic published until end of February 2020.

Polymerases and the Replisome: Machines within Machines

of a collaboration between the synthetic capacities of Massachusetts Institute of Technology multiple polymerases. Two types of polymerases are Cambridge, Massachusetts 02139 required: primases, which start chains, and replicative polymerases, which synthesize the majority of the DNA (Kornberg and Baker, 1992). The replication fork, how-Synthesis of all genomic DNA involves the highly coordi-ev...